Journal: Microbiology Resource Announcements

Article Title: Draft genome sequences from 127 Legionella spp. strains isolated in water systems linked to legionellosis outbreaks

doi: 10.1128/mra.01154-23

Figure Lengend Snippet: Statistics of assembled genomes

Article Snippet: Presumptive positive colonies were further confirmed by qualitative real-time PCR using the IQ-Check screen L. pneumophila Kit (Bio-Rad, Italy) and serologically identified using Legionella rapid latex test Kit (Biolife, Italy) monovalent antisera ( ).

Techniques: Isolation

Journal: Pathogens

Article Title: Occurrence of Uncultured Legionella spp. in Treated Wastewater Effluent and Its Impact on Human Health (SCA.Re.S Project)

doi: 10.3390/pathogens13090786

Figure Lengend Snippet: Rate (%) of positive samples and viable cell concentrations of Legionella spp. and L. pneumophila , collected from the effluent of WWTP, W1 (well 1), and W2 (well 2).

Article Snippet: The extracted DNA (5 μL) from each sample was diluted 10 0 and 10 −1 and subjected to real-time PCR amplification with two different iQ-Check ® Screen Legionella real-time PCR detection kits (Bio-Rad) validated AFNOR (one for Legionella spp.

Techniques:

Journal: Pathogens

Article Title: Occurrence of Uncultured Legionella spp. in Treated Wastewater Effluent and Its Impact on Human Health (SCA.Re.S Project)

doi: 10.3390/pathogens13090786

Figure Lengend Snippet: Average concentrations ± SE of viable Legionella spp. and L. pneumophila in WWTP effluent W1 (well 1) and W2 (well 2) recorded in cold months (November 2022–April 2023) and warm months (May–October 2022).

Article Snippet: The extracted DNA (5 μL) from each sample was diluted 10 0 and 10 −1 and subjected to real-time PCR amplification with two different iQ-Check ® Screen Legionella real-time PCR detection kits (Bio-Rad) validated AFNOR (one for Legionella spp.

Techniques:

Journal: Pathogens

Article Title: Occurrence of Uncultured Legionella spp. in Treated Wastewater Effluent and Its Impact on Human Health (SCA.Re.S Project)

doi: 10.3390/pathogens13090786

Figure Lengend Snippet: Legionella spp.’s composition in water samples assessed by sequencing 16S rRNA gene V3–V4 domain amplicon with Illumina MiSeq (similarity > 97%).

Article Snippet: The extracted DNA (5 μL) from each sample was diluted 10 0 and 10 −1 and subjected to real-time PCR amplification with two different iQ-Check ® Screen Legionella real-time PCR detection kits (Bio-Rad) validated AFNOR (one for Legionella spp.

Techniques: Sequencing, Amplification

Journal: Pathogens

Article Title: Occurrence of Uncultured Legionella spp. in Treated Wastewater Effluent and Its Impact on Human Health (SCA.Re.S Project)

doi: 10.3390/pathogens13090786

Figure Lengend Snippet: Average value (%) of uncultured as-yet-undefined Legionella species.

Article Snippet: The extracted DNA (5 μL) from each sample was diluted 10 0 and 10 −1 and subjected to real-time PCR amplification with two different iQ-Check ® Screen Legionella real-time PCR detection kits (Bio-Rad) validated AFNOR (one for Legionella spp.

Techniques:

Journal: Pathogens

Article Title: Occurrence of Uncultured Legionella spp. in Treated Wastewater Effluent and Its Impact on Human Health (SCA.Re.S Project)

doi: 10.3390/pathogens13090786

Figure Lengend Snippet: ( a ) NCBI BLASTN phylogenetic tree showing the similarities (>97%) between the uncultured Legionella sp. clone BFI-A19-1 (in yellow) and known Legionella species (in green), determined with 16S rDNA sequencing. The scale bar corresponds to 0.02 substitutions per nucleotide position. ( b ) NCBI BLASTN phylogenetic tree showing the similarities (>97%) between uncultured Legionella sp. clone Tag 4-1 (in yellow) and known Legionella species (in green), determined with 16S rRNA sequencing. The scale bar corresponds to 0.02 substitutions per nucleotide position.

Article Snippet: The extracted DNA (5 μL) from each sample was diluted 10 0 and 10 −1 and subjected to real-time PCR amplification with two different iQ-Check ® Screen Legionella real-time PCR detection kits (Bio-Rad) validated AFNOR (one for Legionella spp.

Techniques: Sequencing